Wednesday, May 10, 2006

Getting to work

I am getting to work....watch out proteins, here I come

Differential Label-free Quantitative Proteomic Analysis of Shewanella oneidensis Cultured
under Aerobic and Suboxic Conditions by Accurate Mass and Time Tag Approach
- No email for contact (just address)
- I forget is "Submission Date" for paper or data?
- This did not use isotopes, does that mean there are no modifications (I couldn't find any)
-Assumed conventional ion trap = ESI
-Since this was not a human, did not find much on the eVOC site
- Has data but did not publish

High Expression of Antioxidant Proteins in Dendritic Cells
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Not a great summary of methods
-is [3H] Thymidine incorporation into T cells the treatment labeling?
- ZSPRAY or elctrospray capillary = ESI?
- Has data but did not publish

A Tandem Affinity Tag for Two-step Purification under Fully Denaturing Conditions
-Carboxyl-terminal tagging of Skp1 and Met4 was performed by a PCR-based method as
described previously (11).....Is this the modifications?
-nanoflow reverse phase LC
- what is "dedesalted offline using a C18 Ziptip"
-used Strains—Yeast strains used inthis study are all isogenic to 15Daub, bar1 ura3ns, a derivative ofBF264-15D (9) but I could only find the general "yeast" online
-Has data but did not publish

Phosphate-binding Tag, a New Tool to Visualize Phosphorylated Proteins
--Has data but did not publish
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Is IEF a method of seperation?
- No idea what was used to cleave or what organism was used

Multiplex Analysis of Intracellular Signaling Pathways in Lymphoid Cells by Microbead Suspension Arrays
- I don't think this used Mass Spec
- No published data

Microwave-assisted Protein Preparation and Enzymatic Digestion in Proteomics
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is "ion trap" ESI?

Simultaneous Qualitative and Quantitative Analysis of theEscherichia coli Proteome: A Sweet Tale
-Has data but did not publish
-low energy (MS) and elevated energy (MSE)= MS/MSE ?

Quantitative Proteomic Comparison of Rat Mitochondria from Muscle, Heart, and Liver
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Has data but did not publish
- is DTT a modification?
- Has "methionine oxidation and protein amino-terminal acetylation as variable modifications" but how do I put those in the database

Maturation of Human Neutrophil Phagosomes Includes Incorporation of Molecular Chaperones and Endoplasmic Reticulum Quality Control Machinery
- Has data but did not publish
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for the analysis is says it searched for "carbamidomethylation, modification of peptide
N-terminal glutamine to pyroglutamine, oxidation of methionine, acrylamide-modified cysteine" but doesn't mention them in the methods as modifications

Characterization of an RNA Granule from Developing Brain
- has data but did not publish
-
what is Day 18 Brain for the eVOC
- variable was "oxidation (Met)"
-Difficult article to get much info from

Dynamic Spectrum Quality Assessment and Iterative Computational Analysis of Shotgun Proteomic Data
-
Has data but did not publish
-
carboxyamidomethylation of cysteines and methionine oxidation as variable modifications ?

Proteomic Analysis of Bovine Brain G Protein Has data but did not publish
-
Not much for information

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